rabbit polyclonal anti rad18  (Cell Signaling Technology Inc)

Journal: Medicine

Article Title: Risk of cervical squamous cell carcinoma associated with a single nucleotide polymorphism in the RAD18 gene in the Chinese population and its significance as a predictive biomarker

doi: 10.1097/MD.0000000000044017

Figure Lengend Snippet: RAD18 expression in CSCC with different genetic polymorphisms as determined by immunohistochemistry (40× objective, magnified). (A) rs250403-AA; (B) rs250403-AG; (C) rs250403-GG; (D) rs615967-AA; (E) rs615967-AG; (F) rs615967-GG. The distinct brown coloration is mainly located in the nucleus of the positive cells.

Article Snippet: The sections were firstly incubated with Rabbit anti-RAD18 Polyclonal antibody (1:200, 18333-1-AP, Proteintech), and then incubated with Dako Envision TM Peroxidase (Dako Diagnostica, Hamburg, Germany), and visualized with 3,3’-diaminobenzidine tetrahydrochloride (Dako).

Techniques: Expressing, Immunohistochemistry

Journal: Cell reports

Article Title: Fanconi anemia-associated chromosomal radial formation is dependent on POLθ-mediated alternative end joining

doi: 10.1016/j.celrep.2023.112428

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit polyclonal anti-RAD18 , Bethyl Laboratories , Cat # A301–340A; RRID: AB_937974.

Techniques: Recombinant, Membrane, Plasmid Preparation, Staining, Transfection, Reverse Transcription, Software, Real-time Polymerase Chain Reaction, Microscopy, Cell Counting

Journal: Annals of surgical oncology

Article Title: High RAD18 Expression is Associated with Disease Progression and Poor Prognosis in Patients with Gastric Cancer.

doi: 10.1245/s10434-020-08518-2

Figure Lengend Snippet: FIG. 3 Functional analysis of RAD18 in human GC cell lines. a RAD18 expression was evaluated in the GC cell lines MKN7, MKN74, MKN45, and GCIY by Western blotting. b-Actin was used as the loading control. b RAD18 suppression was evaluated in MKN45 and GCIY cells treated with RAD18 siRNAs by Western

Article Snippet: The sections were then passively cooled to room temperature and incubated in protein block serum-free reagent (DAKO, Carpinteria, CA, USA) for 30 min. Next, the specimens were incubated with a rabbit polyclonal anti-RAD18 antibody (Abcam, Cambridge, UK) at a 1:100 dilution in PBS containing 0.1% bovine serum albumin at 4 C for 24 h. Primary antibody staining was visualized using the Histofine Simple Stain MAX-PO (Multi) Kit (Nichirei, Tokyo, Japan) according to the manufacturer’s instructions.

Techniques: Functional Assay, Expressing, Western Blot, Control

Journal: Annals of surgical oncology

Article Title: High RAD18 Expression is Associated with Disease Progression and Poor Prognosis in Patients with Gastric Cancer.

doi: 10.1245/s10434-020-08518-2

Figure Lengend Snippet: FIG. 4 Analysis of cisplatin chemosensitivity in RAD18-suppressed GC cells. a Expression of RAD18, phospho-H2AX, PCNA, and mUB-PCNA in MKN45, and b in GCIY cells treated with cisplatin for 6 or 24 h using Western blot. b-Actin was used as the loading control (left panel). The IC50 values for cisplatin were determined in GC cells after treatment with cisplatin for 72 h. The IC50 values were

Article Snippet: The sections were then passively cooled to room temperature and incubated in protein block serum-free reagent (DAKO, Carpinteria, CA, USA) for 30 min. Next, the specimens were incubated with a rabbit polyclonal anti-RAD18 antibody (Abcam, Cambridge, UK) at a 1:100 dilution in PBS containing 0.1% bovine serum albumin at 4 C for 24 h. Primary antibody staining was visualized using the Histofine Simple Stain MAX-PO (Multi) Kit (Nichirei, Tokyo, Japan) according to the manufacturer’s instructions.

Techniques: Expressing, Western Blot, Control

Journal: Molecular Cell

Article Title: The ubiquitin ligase RFWD3 is required for translesion DNA synthesis

doi: 10.1016/j.molcel.2020.11.029

Figure Lengend Snippet: RFWD3 ubiquitin ligase activity regulates PCNA ubiquitylation in human cells (A) U2OS cells or U2OS cells expressing Strep-HA-PCNA were transfected with siCtrl or 4 different siRNAs against RFWD3 and either left untreated or treated with UV (30 J/m 2 ). PCNA was recovered under denaturing conditions via Strep-Tactin pull-down and analyzed by immunoblotting with the indicated antibodies. (B) U2OS cells or U2OS/FRT GFP-RFWD3 WT or catalytic inactive (C315A) cells were treated with the indicated siRNAs, transfected with either empty vector (EV) or Strep-HA-PCNA plasmids, and treated with doxycycline to induce expression of RFWD3. UV treatment and protein pull-down were performed as in (A). (C) U2OS cells were transfected with the indicated plasmids and then subjected to Strep-Tactin pull-down as in (A). (D) U2OS/FRT GFP-RFWD3 WT cells were treated with doxycycline and transfected with EV or Strep-HA-PCNA 24 h before lysis in denaturing buffer. Lysates were subjected to Strep-Tactin pull-down in denaturing conditions, washed, and incubated with USP2 (ubiquitin protease) and/or UPL1 (SUMO protease), as indicated. (E) U2OS cells or U2OS/FRT GFP-RFWD3 WT cells were transfected with either control (siCtrl) or RAD18 siRNAs. After 48 h, cells were transfected with either EV or Strep-HA-PCNA plasmids and treated with doxycycline to induce the expression RFWD3 WT. Then, cells were treated with UV for 4 h and processed for Strep-Tactin pull-down as described in (A). The asterisk denotes a non-specific band.

Article Snippet: Rabbit polyclonal antibody anti RAD18 , Bethyl Laboratories , Cat#A301-340A; RRID: AB_937974.

Techniques: Ubiquitin Proteomics, Activity Assay, Expressing, Transfection, Western Blot, Plasmid Preparation, Lysis, Incubation, Control

Journal: Molecular Cell

Article Title: The ubiquitin ligase RFWD3 is required for translesion DNA synthesis

doi: 10.1016/j.molcel.2020.11.029

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal antibody anti RAD18 , Bethyl Laboratories , Cat#A301-340A; RRID: AB_937974.

Techniques: Ubiquitin Proteomics, Recombinant, Mutagenesis, Staining, Magnetic Beads, Transfection, Sequencing, Software